Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages over ...

A highly efficient and reliable fluorescent probe for PCR DNA amplification techniques and DNA analysis in hybridization experiments has been developed. This technology will enable the development of ...

Scorpions ™ Primers integrate primer and probe into a single bifunctional molecule. Unlike dual-labeled probes and Molecular Beacons, Scorpions Primers combine primer and probe in one molecule, with ...

Gene expression experiments measure changes in RNA transcript levels to compare how genes are up- or down-regulated across ...

The real-time polymerase chain reaction( PCR) has become a key tool for molecular assay development, expanding into applications as diverse as in vitro diagnostics (IVDs), food safety testing, and ...

The changing technology landscape will affect the BioWatch program. This chapter comments on additional assay performance considerations that arise from the use of multiplexed real-time polymerase ...

Ten PCR assays targeting five biological agents (Bacillus anthracis, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis) were incorporated onto Array Cards. A ...